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Alveole Primo (alveole)

Overview

Alveole Primo allows precise and rapid way to pattern proteins. This tool uses UV laser (375nm) which is used to illuminate your pattern onto the substrate. A photoactive reagent PLPP with the UV illumination enables creation of the pattern. Proteins can then selectively adhere into the desired pattern. The minimum resolution of this tool is about 1.5um

Cleanliness: 
Flexible

Processing Technique(s)

Lab Facility, Location, and NEMO Information

Lab Facility: 
(SNF Exfab)
Location: 
SNF Exfab Paul G Allen 151 Ocean
NEMO Area (ExFab by room): 
ExFab: 151 Ocean
NEMO ID: 
alveole

Training and Maintenance

Lab Facility: 
SNF Exfab
Training Charges: 
2.00 hours
Primary Trainer: 
Swaroop Kommera
Primary Maintenance: 
Swaroop Kommera

Steps to become a tool user

  1. Become a member of SNF.
  1. Study the relevant operating procedures:
  2. Shadowing is required. Contact a qualified lab member of the tool to arrange to ‘shadow’. It would be best to find someone who has used the system often. If you don’t know of anyone, you may check reservations or SNF usage to find a qualified user. We recommend that you be with the lab member for the full time while operating the tool and ask lots of questions during the shadowing. You may have to shadow a qualified user more than one time to be comfortable with the tool. Please follow the instructions on this form: Shadowing at SNF
  3. Contact the primary trainer: Swaroop Kommera